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However, since 1998, there have been separate and repeated incursions of bluetongue into Europe and 6 of the 24 different serotypes (BTV-1, -2, -4, -8, -9 and -16) have been introduced into.
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BTV serotypes 1, 2, 4, 8, and 16 of Italian isolates were kindly provided by Dr. Savini (Istituto Zooprofilattico Sperimentale dell'Abruzzo et del Molise "G. Caporale", Italy). These viruses were propagated in Vero cells. To isolate single plaques from endpoint dilutions an overlay with 1% agarose was placed on an infected cell monolayer.
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In this study, we developed and evaluated the beta-propiolactone inactivated bivalent bluetongue virus (BTV) serotypes 4 and 16 vaccine delivered with Montanide™ ISA-71VG adjuvant. The safety, stability and immunological profile of the fresh and after three years of long-term storage of the vaccine.
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ELISA Results on Naïve or BTV-1, 2, 8, 9 or 16 Infected or Vaccinated Animals. A vast majority of the 319 BTV-naïve ruminant sera had an OD of less than 0.1. Only three sera (1%) had an OD between 0.15 and 0.25 (Figure 2 A). All seven sera representing the seven serotypes of EHD were negative. Similar results were obtained in the presence of.
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Viremia was analyzed by plaque assay (for BTV-1, BTV-4, and BTV-8) or measured by real-time RT-qPCR specific for BTV segment 5 (for BTV-4M and BTV-16, unable to form lysis plaques in cell culture). The real-time RT-qPCR specific for BTV segment 5 was performed as described by Toussaint et al. ( 47 ), and mouse blood samples containing different.
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Abstract. Since 1998, bluetongue virus (BTV) serotypes 1, 2, 4, 6, 8, 9, 11 and 16 have spread throughout Europe. In 2006, BTV serotype 8 (BTV-8) emerged unexpectedly in northern Europe throughout a region including Belgium, France, Germany, Luxembourg and the Netherlands. In the following year, it spread rapidly throughout the rest of Europe.
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Six BTV serotypes (BTV-1, BTV-2, BTV-4, BTV-8, BTV-9, and BTV-16) are present in those regions coming from three different epidemiological systems. The first one is located in the eastern part of the Mediterranean Sea and the serotypes involved (BTV-1, BTV-4, BTV-9, and BTV-16) seem to come from the Near, Middle, and Far East..
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In turn, one sample from 2004 given positive for BTV-16 resulted positive also for BTV-4; three samples from 2002 given positive for serotypes -2 and -9 (C T values 27.1, 28.9 and 28.9) were identified by the BTV CodeSet only as BTV-9; one tissue sample given positive from 2015 for BTV-4 (C T 27.0 by qPCR NS3) was identified by the BTV CodeSet.
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In Lebanon, BTV-1, BTV-4, BTV-6, BTV-8, BTV-16 and BTV-24 were identified in 2011 (El Hage et al. 2013). In other west Asian countries, BT has been reported from Iraq, Iran, Jordan, Oman, Saudi Arabia, Syria, Turkey and Yemen; the newer serotype, BTV-26, has been isolated from Kuwait (Maan et al. 2011).
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Sheep were then challenged by subcutaneous injection of either BTV-16 (2 groups) or BTV-8 (5 groups). Taken together, 24/25 sheep from the 4 experimental groups developed detectable antibodies against the vaccinated viruses. Furthermore, sheep that received heterologous vaccines showed significantly reduced viraemia and clinical scores for BTV.
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A Seg-2 serotype-specific real-time RTPCR identified BTV-4, BTV-8, BTV-10 and BTV-16. This finding represents the first detection of BTV-8, BTV10 and BTV-16 in Brazil and, additionally, the first detection of BTV genomes in semen from bulls in the Country. RNA from multiple BTV-serotypes was detected in two individual semen samples suggesting.
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About BTV PUR ®. Active immunisation of sheep to prevent viraemia and to reduce clinical signs caused by bluetongue virus serotypes 1, 2, 4 and/or 8 (combination of maximum 2 serotypes). Active immunisation of cattle to prevent viraemia caused by bluetongue virus serotypes 1, 2, 4 and/or 8, and to reduce clinical signs caused by bluetongue.
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The analytical sensitivity of the Seg-2 type-specific assays for the European serotypes (BTV-1, -2, -4, -6, -8, -9, -11, and -16) were assessed using a dilution series of the quantified dsRNA genome of reference strains of each BTV serotype (as listed in S1 Table). Additionally, this selection of the assays for the European serotypes were also.
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In 2006, BTV serotype 8 (BTV-8). 8, 12, and 16 post infection were processed for qRT-PCR. Two of the three pools (n = 5) were processed from C. sonorensis collected on days 2, 6, 10, 14, and 18 dpi. Some pools were reserved for potential individual analysis. For processing, each pool was manually homogenized with a sterile pestle in 250 µL.
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Viral strains. We used the BTV strains Khuroson-40/13/4 (BTV-4) and RT/RIBSP40/13/16 (BTV-16) (Sametova et al. 2013), which were obtained in lyophilized form from the laboratory of the "Collection of Microorganisms" at the Research Institute for Biological Safety Problems (RIBSP) and refreshed in Vero cells.Both strains were isolated individually by serial passages in chicken embryos (to.
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The Bluetongue virus serotype 8 (BTV-8) strain, which emerged in Europe in 2006, had an unusually high ability to cause foetal infection in pregnant ruminants. Other serotypes of BTV had already been present in Europe for more than a decade, but transplacental transmission of these strains had never.