PT FAD
Initially, we investigated the capability of FAD (flavin adenine dinucleotide) to act as a catalyst for the photoactivation of two classes of anticancer metal complexes, namely octahedral Pt IV and Ru II -arene piano-stool complexes. Complexes 1-3 are prodrugs of cisplatin and carboplatin, 13 and complexes 4 and 5 are photoactivatable scaffolds capable of generating reactive Ru−OH 2.
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In a very large superfamily of dinucleotide binding enzymes, the domains that bind a dinucleotide such as FAD, NAD, and NADP share a common super-secondary structure that is partly composed of a series of alternating beta strand (β) and alpha helical (α) segments wherein the β-strands are hydrogen bonded forming a central β-sheet with helices on either side.
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PT FAD
The oxidation of Pt on Nafion shows striking differences to the case of Pt on FAD discussed above. Figure 5a shows the stepwise oxidation of a single layer of Nafion-supported nanoparticles. The onset potential for oxide formation lies between 0.9 V RHE and 1.15 V RHE (in agreement with the literature ), roughly 0.2 V lower than for Pt/FAD.
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Here, we study the surface restructuring and Pt dissolution mechanisms during oxidation/reduction for the case of Pt(100) in 0.1 M HClO4 by combined operando high-energy surface X-ray diffraction.
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FAD and its derivatives were separated by 6 min of isocratic flow in 15% acetonitrile/water buffered with 0.1% (v/v) formic acid, ensued by a linear gradient from 15 to 53% acetonitrile over 24.
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Thus, we identify sulfate, Br-species, and O-species as the adsorbates in the Pt-FAD system and the N-functionalities and sulfate as bulk electrolyte species. An analogous analysis on Nafion is presented in Figure S10 in the ESI. For both FAD and Nafion, we observe an intensity increase for membrane species during anodization.
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Cofactor regeneration using enzymes has been considered a favorable system and has been the only one applied practically at industrial scale. One of the earliest examples demonstrating enzymatic NAD(P)H preparation from NAD(P) + was published in 1957 when ethanol and alcohol dehydrogenase (ADH) were used by Rafter and Colowick. 24 Enzymatic approaches for regeneration offer excellent.
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Pt NPs can catalyze the reduction of not only FMN but also FAD and coenzyme Q. Pd NPs also exhibited catalytic activities (Figs. 4g and s24-s27). For Au, Ru, Rh, and Ir NPs, O 2 was hard to exhaust.
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Management Flight (CEMF); Force Activity Designator (FAD) Management; validating War Reserve Materiel (WRM) and Readiness Spares Package (RSP) funding requirements. (AMC) This publication has been updated to reflect new information that is specific to AMC and should be reviewed in its entirety.
